The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.<\/p>\n","protected":false},"excerpt":{"rendered":"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) […]<\/p>\n","protected":false},"featured_media":83045,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[873],"product_cat":[1228],"product_tag":[],"class_list":{"0":"post-83043","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-c2","7":"product_cat-hamster-ruo-elisa","8":"pa_size-48t","9":"pa_size-96t","11":"first","12":"instock","13":"sale","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/83043","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/83045"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=83043"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=83043"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=83043"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=83043"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}