GelRed\u00ae is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed\u00ae Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed\u00ae dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Download the product protocol on this page for a comparison of prestain, precast, and post-stain protocols for GelRed\u00ae, and to find the recommended method for your application.\u00a0\u00a0The 6X GelRed\u00ae Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.\u00a0\u00a0GelRed\u00ae and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed\u00ae without changing your existing imaging system. In addition, GelRed\u00ae is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed\u00ae is compatible with downstream applications such as sequencing and cloning. GelRed\u00ae is efficiently removed from DNA by commercial gel extraction kits or by phenol\/chloroform extraction and ethanol precipitation.<\/p>\n
Protect from light;<\/p>\n
GelRed\u00ae is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed\u00ae Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed\u00ae dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates […]<\/p>\n","protected":false},"featured_media":714857,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1722,1677,1723],"product_tag":[],"class_list":{"0":"post-717769","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-dna-binding-dyes","8":"product_cat-biotium-dna-gel-stains","9":"product_cat-dna-gel-stains-related-products","11":"first","12":"instock","13":"taxable","14":"shipping-taxable","15":"purchasable","16":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717769","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714857"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717769"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717769"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717769"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717769"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}