{"id":717711,"date":"2026-05-05T03:12:58","date_gmt":"2026-05-05T03:12:58","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717711"},"modified":"2026-05-05T03:12:58","modified_gmt":"2026-05-05T03:12:58","slug":"hoechst-33258","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/hoechst-33258\/","title":{"rendered":"Hoechst 33258"},"content":{"rendered":"<p>Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Biotium also offers Hoechst 33258, 10 mg\/ml in dH2O (#40044). Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug\/mL, with no wash step required.\u00a0 <\/p>\n<ul>\u00a0 <\/p>\n<li>&amp;lamba;<sub>Ex<\/sub>\/&amp;lamba;<sub>Em <\/sub>(with DNA) = 352\/461 nm<\/li>\n<p>\u00a0 <\/p>\n<li>Pale yellow solid soluble in water up to 2% (w\/v)<\/li>\n<p>\u00a0 <\/p>\n<li>Store at -20\u00b0C and protect from light<\/li>\n<p>\u00a0 <\/p>\n<li>C<sub>25<\/sub>H<sub>27<\/sub>Cl<sub>3<\/sub>N<sub>6<\/sub>O.5H<sub>2<\/sub>O<\/li>\n<p>\u00a0 <\/p>\n<li>MW: 623.96<\/li>\n<p>\u00a0 <\/ul>\n<h3>Handling<\/h3>\n<p>Store with desiccant; Protect from light<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Biotium also offers Hoechst 33258, 10 mg\/ml in dH2O (#40044). Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, [&hellip;]<\/p>\n","protected":false},"featured_media":714846,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1722,1687,1714,1668,1705,1749],"product_tag":[],"class_list":{"0":"post-717711","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-dna-binding-dyes","8":"product_cat-biotium-fluorescence-microscopy","9":"product_cat-biotium-nuclear-counterstains","10":"product_cat-nuclear-stains-dna-rna-dyes","11":"product_cat-biotium-other-bacterial-stains","12":"product_cat-biotium-super-resolution-imaging","14":"first","15":"instock","16":"taxable","17":"shipping-taxable","18":"purchasable","19":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717711","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714846"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717711"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717711"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717711"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717711"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}