{"id":717701,"date":"2026-05-05T03:12:54","date_gmt":"2026-05-05T03:12:54","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717701"},"modified":"2026-05-05T03:12:54","modified_gmt":"2026-05-05T03:12:54","slug":"ethidium-monoazide-bromide-ema","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/ethidium-monoazide-bromide-ema\/","title":{"rendered":"Ethidium Monoazide Bromide (EMA)"},"content":{"rendered":"<p>Ethidium monoazide bromide (EMA) is a fluorescent nucleic acid stain with a photoaffinity label. The dye, after photolysis, binds covalently to nucleic acids.The dye has been used to footprint drug binding sites on DNA, to modify plasmid DNA, and for labeling yeast cells for leukocyte phagocytosis assays. A particularly useful application of the dye is to label dead cells in a mixed population of live and dead cells. Because EMA\u00a0is relatively impermeant to live cells, it selectively binds DNA in dead cells. Photolysis renders the dead cell DNA covalently labeled with the dye. One can then wash and fix the cell preparation for analysis by microscopy, fluorescence plate reader or flow cytometry. The major advantage of this method is that researchers can avoid extensive manipulation of live pathogenic organisms. EMA\u00a0also has been used to differentiate between viable and dead bacteria by 5&#8242;-nuclease PCR.\u00a0 <\/p>\n<li>Ex (pH 3) = 458 nm<\/li>\n<p>\u00a0 <\/p>\n<li>&amp;lamba;<sub>Ex<\/sub>\/&amp;lamba;<sub>Em<\/sub> (after photocrosslinking to nucleic acid) = 510\/600 nm<sup>6<\/sup><\/li>\n<p>\u00a0 <\/p>\n<li>Orange solid soluble in DMF, or ethanol<\/li>\n<p>\u00a0 <\/p>\n<li>Store desiccated at 4<sup>o<\/sup>C or -20<sup>o<\/sup>C and protect from light, especially in solution<\/li>\n<p>\u00a0 <\/p>\n<li>C<sub>21<\/sub>H<sub>18<\/sub>BrN<sub>5<\/sub><\/li>\n<p>\u00a0 <\/p>\n<li>MW: 420<\/li>\n<p>\u00a0 <\/p>\n<li>[58880-05-0]<\/li>\n<p>\u00a0 <\/ul>\n<p>\u00a0 \u00a0For viability PCR applications, Biotium also offers propidium monoazide iodide (PMA), as well as our new and improved viability PCR dye, PMAxx\u2122. PMA and PMAxx\u2122 are less membrane permeant than EMA and therefore more selective for dead cell labeling for qPCR quantitation of viable bacteria.\u00a0 \u00a0 For photoactivation of PMA, PMAxx\u2122 or EMA dye, we recommend the use of Biotium&#8217;s PMA-Lite\u2122 LED Photolysis Device (for tubes) or Glo-Plate\u2122 Blue LED Illuminator (for plates), which are designed to conduct photolysis under controlled conditions.\u00a0 <\/p>\n<ul>\n<h3>Handling<\/h3>\n<p>Protect from light;<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Ethidium monoazide bromide (EMA) is a fluorescent nucleic acid stain with a photoaffinity label. The dye, after photolysis, binds covalently to nucleic acids.The dye has been used to footprint drug binding sites on DNA, to modify plasmid DNA, and for labeling yeast cells for leukocyte phagocytosis assays. A particularly useful application of the dye is [&hellip;]<\/p>\n","protected":false},"featured_media":715251,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1722,1730,1706],"product_tag":[],"class_list":{"0":"post-717701","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-dna-binding-dyes","8":"product_cat-biotium-reactive-dna-rna-binding-dyes","9":"product_cat-biotium-viability-pcr-reagents","11":"first","12":"instock","13":"taxable","14":"shipping-taxable","15":"purchasable","16":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717701","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/715251"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717701"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717701"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717701"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717701"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}