EvaGreen\u00ae Dye, 2000X \u00a0in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.\u00a0\u00a0Note: for most qPCR applications, we recommend our ready-to-use\u00a0Forget-Me-Not\u2122 EvaGreen\u00ae qPCR Master Mixes or EvaGreen\u00ae Dye, 20X \u00a0in water.\u00a0\u00a0EvaGreen\u00ae dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR\u00ae Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen\u00ae dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen\u00ae dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR\u00ae Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.\u00a0\u00a0The unique properties of EvaGreen\u00ae dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR\u00ae Green I, EvaGreen\u00ae dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen\u00ae dye can be used at a much higher dye concentration than SYBR\u00ae Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.\u00a0\u00a0EvaGreen\u00ae dye and applications are covered under granted and pending US and international patents.<\/p>\n
Protect from light;<\/p>\n
EvaGreen\u00ae Dye, 2000X \u00a0in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.\u00a0\u00a0Note: for most qPCR applications, we recommend our ready-to-use\u00a0Forget-Me-Not\u2122 EvaGreen\u00ae qPCR Master Mixes or EvaGreen\u00ae Dye, 20X \u00a0in water.\u00a0\u00a0EvaGreen\u00ae dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR […]<\/p>\n","protected":false},"featured_media":714973,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1722,1755,1731],"product_tag":[],"class_list":{"0":"post-717513","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-dna-binding-dyes","8":"product_cat-biotium-qpcr-dyes","9":"product_cat-biotium-qpcr-reagents","11":"first","12":"instock","13":"taxable","14":"shipping-taxable","15":"purchasable","16":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717513","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714973"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717513"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717513"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717513"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717513"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}