EvaGreen\u00ae dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR\u00ae Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen\u00ae dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen\u00ae dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR\u00ae Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.\u00a0\u00a0The unique properties of EvaGreen\u00ae dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR\u00ae Green I, EvaGreen\u00ae dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen\u00ae dye can be used at a much higher dye concentration than SYBR\u00ae Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.\u00a0\u00a0See our ready-to-use Forget-Me-Not\u2122 EvaGreen\u00ae qPCR master mixes for sensitive, competitively-priced qPCR reagents. We also offer Thiazole Green, 1000X in DMSO, which is identical to SYBR\u00ae Green I.\u00a0\u00a0EvaGreen dye and applications are covered by granted US and international patents.\u00a0\u00a0<\/p>\n
\u00a0\u00a0Low PCR inhibition<\/b>\u00a0Exhibits much less PCR inhibition than SYBR\u00ae Green I via a smart “release-on-demand” DNA-binding technology\u00a0\u00a0Highly Sensitive<\/b>\u00a0Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals\u00a0\u00a0Nonmutagenic and noncytotoxic<\/b>\u00a0Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes\u00a0\u00a0Compatible with fast PCR protocols<\/b>\u00a0Minimal interference to PCR makes it possible to significantly shorten the chain extension time\u00a0\u00a0Compatible with multiplex PCR<\/b>\u00a0No dye migration from amplicon to amplicon when used at the recommended concentration\u00a0\u00a0Unsurpassed thermal stability, chemical stability and photostability<\/b>\u00a0No detectable dye decomposition in PCR buffer at 95-100\u00b0C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles\u00a0\u00a0Spectrally similar to SYBR\u00ae Green I<\/b>\u00a0Compatible with all major brand qPCR instruments and enzyme systems<\/p>\nHandling<\/h3>\n
Protect from light;<\/p>\n
EvaGreen\u00ae dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye […]<\/p>\n","protected":false},"featured_media":714572,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1722,1755,1731],"product_tag":[],"class_list":{"0":"post-717486","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-dna-binding-dyes","8":"product_cat-biotium-qpcr-dyes","9":"product_cat-biotium-qpcr-reagents","10":"pa_size-1-ml","11":"pa_size-5-x-1-ml","13":"first","14":"instock","15":"taxable","16":"shipping-taxable","17":"purchasable","18":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717486","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714572"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717486"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717486"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717486"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717486"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}