{"id":717285,"date":"2026-05-05T03:09:35","date_gmt":"2026-05-05T03:09:35","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717285"},"modified":"2026-05-05T03:09:35","modified_gmt":"2026-05-05T03:09:35","slug":"nucview-488-and-reddot-2-apoptosis-necrosis-kit-100-assays","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/nucview-488-and-reddot-2-apoptosis-necrosis-kit-100-assays\/","title":{"rendered":"NucView\u00ae 488 and RedDot\u2122 2 Apoptosis &amp; Necrosis Kit (100 assays)"},"content":{"rendered":"<p>NucView\u00ae488 and RedDot\u2122 Apoptosis\/Necrosis Assay Kit contains NucView\u00ae488 Caspase-3 Substrate for detection of caspase-3\/7 activity. The far-red dead-cell stain RedDot\u2122 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.\u00a0\u00a0In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView\u00ae488 Caspase-3 Substrate can be used to detect caspase-3\/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3\/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3\/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView\u00ae488 Caspase-3 Substrate allows detection caspase-3\/7 activity and visualization of morphological changes in the nucleus during apoptosis.\u00a0\u00a0RedDot\u2122 is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. RedDot\u2122 has far-red emission at 695 nm for detection in the Cy\u00ae channel, well-separated from the green fluorescence of NucView\u00ae488.\u00a0The excitation maximum of RedDot\u2122 is 665 nm, but it can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.\u00a0\u00a0Note: while NucView\u00ae488 staining is formaldehyde-fixable and compatible with subsequent immunostaining, fixation after staining with RedDot\u2122 is not recommended because it can result in increased background staining of healthy cells.\u00a0\u00a0See our full selection of NucView\u00ae substrates and kits.\u00a0\u00a0NucView\u00ae enzyme substrate technology is covered by U.S. patents.\u00a0\u00a0Cy5 is a registered trademark of GE Healthcare.<\/p>\n<h3>Handling<\/h3>\n<p>Protect from light;<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>NucView\u00ae488 and RedDot\u2122 Apoptosis\/Necrosis Assay Kit contains NucView\u00ae488 Caspase-3 Substrate for detection of caspase-3\/7 activity. The far-red dead-cell stain RedDot\u2122 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.\u00a0\u00a0In contrast [&hellip;]<\/p>\n","protected":false},"featured_media":714606,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1647,1685,1660,1683],"product_tag":[],"class_list":{"0":"post-717285","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-apoptosis-assays","8":"product_cat-biotium-flow-cytometry","9":"product_cat-biotium-nucview-caspase-3-substrates","10":"product_cat-biotium-protease-substrates","12":"first","13":"instock","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717285","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714606"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717285"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717285"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717285"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717285"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}