{"id":717269,"date":"2026-05-05T03:09:30","date_gmt":"2026-05-05T03:09:30","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717269"},"modified":"2026-05-05T03:09:30","modified_gmt":"2026-05-05T03:09:30","slug":"viafluor-cfse-cell-proliferation-kit","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/viafluor-cfse-cell-proliferation-kit\/","title":{"rendered":"ViaFluor\u00ae CFSE Cell Proliferation Kit"},"content":{"rendered":"<p>ViaFluor\u00ae \u00a0SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. Labeling is covalent, so it withstands fixation and permeabilization for subsequent immunostaining.\u00a0\u00a0The membrane permeable compound is non-fluorescent until it enters viable cells, where it is hydrolyzed by cytoplasmic esterase enzymes to releases the fluorescent amine-reactive dye. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell, while excess unreacted dye is washed away.\u00a0 Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining.\u00a0 Thus, cell proliferation dyes can be used to track multiple cell divisions of cells grown in culture or injected <em>in vivo<\/em> after labeling with the ViaFluor\u00ae SE dye.\u00a0\u00a0Kit Components:\u00a0\u00a0<\/p>\n<ul>\u00a0\u00a0 <\/p>\n<li>10 lyophilized dye vials (full size kits)<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li>1 lyophilized dye vial (trial size kits)<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li>1 vial\u00a0of anhydrous DMSO for preparing stock solutions<\/li>\n<p>\u00a0\u00a0<\/ul>\n<p>\u00a0\u00a0When used at 1 uM in 1 mL of cells, at a cell density of 1 x 10<sup>6<\/sup> cells mL, each dye vial can be used for 90-100 labelings; the final number of assays that can be performed per kit depends on the dye concentration used.\u00a0\u00a0ViaFluor\u00ae kits come with 3 dye options:\u00a0\u00a0<\/p>\n<ul>\u00a0\u00a0 <\/p>\n<li>ViaFluor\u00ae CFSE (also known as CFDA-SE) can be detected in the FITC channel.<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li>ViaFluor\u00ae 488 SE, an improved alternative to CFSE; detected in the FITC channel.<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li>ViaFluor\u00ae 405 SE for the violet laser; can be detected in the Pacific Blue\u00ae channel.<\/li>\n<p>\u00a0\u00a0<\/ul>\n<p>\u00a0\u00a0ViaFluor\u00ae CFSE can be used as a cytoplasmic stain in yeast. However ViaFluor\u00ae 405 SE does not stain well in yeast.<\/p>\n<h3>Handling<\/h3>\n<p>Store with desiccant; Protect from light<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>ViaFluor\u00ae \u00a0SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. 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