{"id":717256,"date":"2026-05-05T03:09:24","date_gmt":"2026-05-05T03:09:24","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717256"},"modified":"2026-05-05T06:29:35","modified_gmt":"2026-05-05T06:29:35","slug":"steady-luc-firefly-hts-assay-kit-120-assays","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/steady-luc-firefly-hts-assay-kit-120-assays\/","title":{"rendered":"Steady-Luc\u2122 Firefly HTS Assay Kit, 120 Assays"},"content":{"rendered":"<p>Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.\u00a0\u00a0Biotium&#8217;s Steady-Luc\u2122 HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.\u00a0\u00a0Glow-type luciferase assays like Steady-Luc\u2122 have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.\u00a0Biotium also offers Steady-Luc\u2122 Firefly HTS Assay Kit (Lyophilized), with lyophilized assay buffer for convenient room temperature shipping and storage at -20\u00b0C.\u00a0\u00a0<\/p>\n<p><strong>Kit Components: <\/strong><\/p>\n<p>\u00a0\u00a0<\/p>\n<ul>\u00a0\u00a0 <\/p>\n<li>D-Luciferin<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li>Steady-Luc Firefly Assay Buffer<\/li>\n<p>\u00a0\u00a0<\/ul>\n<p>\u00a0\u00a0<strong>Features:<\/strong>\u00a0\u00a0<\/p>\n<ul>\u00a0\u00a0 <\/p>\n<li><b>Simple:<\/b> single step homogeneous assay.<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li><strong>Glow kinetics:<\/strong> luminescence half life of about 3 hours.<\/li>\n<p>\u00a0\u00a0 <\/p>\n<li><b>Economic:<\/b> save up to 45% compared to other brands.<\/li>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. [&hellip;]<\/p>\n","protected":false},"featured_media":714949,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1700],"product_tag":[],"class_list":{"0":"post-717256","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-luciferase-reporter-gene-assays","8":"pa_size-kit-large","9":"pa_size-kit-lot-1","10":"pa_size-kit-lot-2","11":"pa_size-kit-lot-3","12":"pa_size-kit-small","13":"pa_size-kit-standard","14":"pa_size-kit-trial","16":"first","17":"instock","18":"taxable","19":"shipping-taxable","20":"purchasable","21":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717256","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714949"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717256"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717256"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717256"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717256"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}