{"id":717231,"date":"2026-05-05T03:09:16","date_gmt":"2026-05-05T03:09:16","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717231"},"modified":"2026-05-05T06:26:46","modified_gmt":"2026-05-05T06:26:46","slug":"viability-cytotoxity-assay-kit-for-animal-live-dead-cells-300-assays","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/viability-cytotoxity-assay-kit-for-animal-live-dead-cells-300-assays\/","title":{"rendered":"Viability\/Cytotoxity Assay Kit for Animal Live &amp; Dead Cells (300 assays)"},"content":{"rendered":"<p>The Viability\/Cytotoxicity Assay Kit for Animal Live &amp; Dead Cells provides green\/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells. The esterase substrate calcein AM stains live cells green, while the membrane-impermeable DNA dye Ethidium homodimer III (EthD-III) stains dead cells red. The kit is suitable for detection using fluorescence microscopy, fluorescence microplate reader, or flow cytometry. The assay principles are general and applicable to most eukaryotic cell types, including adherent cells and certain tissues, but not to bacteria or yeast. This fluorescence-based method of assessing cell viability can be used in place of trypan blue exclusion, 51Cr release, and similar methods for determining cell viability and cytotoxicity. EthD-III (#40050 and #40051) is a proprietary DNA stain developed by Biotium to be a superior alternative to the spectrally similar dye Ethidium homodimer I (EthD-I). EthD-III has higher DNA binding affinity and thus brighter fluorescence (30% brighter than EthD-I).\u00a0\u00a0Spectral Properties\u00a0Calcein (end product after hydrolysis of calcein AM):\u00a0Ex\/Em: 494\/517 nm (pH 8)\u00a0\u00a0Ethidium homodimer III (EthDIII):\u00a0Ex\/Em: 530\/620 nm (with DNA)<\/p>\n<h3>Handling<\/h3>\n<p>Protect from light;<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Viability\/Cytotoxicity Assay Kit for Animal Live &amp; Dead Cells provides green\/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells. The esterase substrate calcein AM stains live cells green, while the membrane-impermeable DNA dye [&hellip;]<\/p>\n","protected":false},"featured_media":714834,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1685,1649],"product_tag":[],"class_list":{"0":"post-717231","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-flow-cytometry","8":"product_cat-proliferation-viability-assays","9":"pa_size-kit","10":"pa_size-kit-trial","12":"first","13":"instock","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717231","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714834"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717231"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717231"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717231"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717231"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}