{"id":717062,"date":"2026-05-05T03:07:46","date_gmt":"2026-05-05T03:07:46","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=717062"},"modified":"2026-05-05T03:07:46","modified_gmt":"2026-05-05T03:07:46","slug":"trueblack-lipofuscin-autofluorescence-quencher-20x-in-dmf","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/trueblack-lipofuscin-autofluorescence-quencher-20x-in-dmf\/","title":{"rendered":"TrueBlack\u00ae Lipofuscin Autofluorescence Quencher, 20X in DMF"},"content":{"rendered":"<p><strong>Features:<\/strong>_x000D_\u00a0<\/p>\n<ul>_x000D_\u00a0 \t<\/p>\n<li>Eliminates lipofuscin autofluorescence<\/li>\n<p>_x000D_\u00a0 \t<\/p>\n<li>Reduces autofluorescence from non-lipofuscin sources<\/li>\n<p>_x000D_\u00a0 \t<\/p>\n<li>Doesn&#8217;t cause high background, unlike Sudan Black B<\/li>\n<p>_x000D_\u00a0 \t<\/p>\n<li>Can be used before or after immunofluorescence staining<\/li>\n<p>_x000D_\u00a0 \t<\/p>\n<li>Clears the way for fluorescence imaging of human and aged animal tissues<\/li>\n<p>_x000D_\u00a0<\/ul>\n<p>_x000D_\u00a0TrueBlack\u00ae Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy, and accumulate in a variety of cell and tissue types with age. Consequently, imaging of specific immunofluorescence signal in certain adult human tissues or aged animal tissues can be virtually impossible unless methods are employed to quench or mask lipofuscin fluorescence._x000D_\u00a0_x000D_\u00a0Traditionally, Sudan Black B has been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However, while it masks the autofluorescence from lipofuscin, Sudan Black B also introduces uniform non-specific background fluorescence in the red and far-red channels, limiting the use of fluorescent dyes in those wavelengths. Now Biotium has developed TrueBlack\u00ae as a superior alternative to Sudan Black B. TrueBlack\u00ae quenches lipofuscin fluorescence with far less increase in red\/far-red background fluorescence. TrueBlack\u00ae treatment can be performed before or after immunostaining. It is rapid, simple, and has minimal effect on signal from fluorescent antibodies or nuclear counterstains, thus preserving the signal-to-noise ratio of the immunostaining._x000D_\u00a0_x000D_\u00a0TrueBlack\u00ae effectively eliminates lipofuscin autofluorescence in tissues like human and mouse brain and retina.\u00a0 TrueBlack\u00ae also can reduce autofluorescence from other sources, such as collagen, elastin, red blood cells, and general background fluorescence. It is not as effective at quenching these sources of autofluorescence as it is for lipofuscin, but it can improve background in human and non-human tissue types. TrueBlack\u00ae also has been used to quench background fluorescence on polycarbonate filters used as cell supports for imaging cytometry._x000D_<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Features:_x000D_\u00a0 _x000D_\u00a0 Eliminates lipofuscin autofluorescence _x000D_\u00a0 Reduces autofluorescence from non-lipofuscin sources _x000D_\u00a0 Doesn&#8217;t cause high background, unlike Sudan Black B _x000D_\u00a0 Can be used before or after immunofluorescence staining _x000D_\u00a0 Clears the way for fluorescence imaging of human and aged animal tissues _x000D_\u00a0 _x000D_\u00a0TrueBlack\u00ae Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for [&hellip;]<\/p>\n","protected":false},"featured_media":714622,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1651,1687],"product_tag":[],"class_list":{"0":"post-717062","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-blocking-buffers-background-reducers","8":"product_cat-biotium-fluorescence-microscopy","10":"first","11":"instock","12":"taxable","13":"shipping-taxable","14":"purchasable","15":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/717062","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714622"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=717062"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=717062"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=717062"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=717062"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}