{"id":715947,"date":"2026-05-05T02:43:13","date_gmt":"2026-05-05T02:43:13","guid":{"rendered":"https:\/\/immunomart.com\/?post_type=product&p=715947"},"modified":"2026-05-05T02:43:13","modified_gmt":"2026-05-05T02:43:13","slug":"evaez-fluorometric-dna-polymerase-activity-assay-200-rxn","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/evaez-fluorometric-dna-polymerase-activity-assay-200-rxn\/","title":{"rendered":"EvaEZ\u2122 Fluorometric DNA Polymerase Activity Assay (200 rxn)"},"content":{"rendered":"<p>EvaEZ\u2122 Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen\u00ae dye together with a primed template, dNTPs, and MgCl<sub>2<\/sub> in a Tris buffer system. In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind EvaGreen dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of polymerase. The assay was developed for the measurement of Taq DNA polymerase activity. It can also be used for other DNA polymerases such as Pfu, Vent, Phusion\u00ae, Bst, Phi29, MMLV, AMV, SuperScript\u00ae, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I. The activity assay can be conducted at temperatures from 4\u00b0C to 75\u00b0C.<\/p>\n<h3>Handling<\/h3>\n<p>Protect from light;<\/p>\n<div style=\"margin:18px 0 4px;padding:11px 14px;background:#fdf4f4;border:1px solid #e6bdbd;border-left:3px solid #c41e3a;border-radius:4px;font-size:13px;line-height:1.55;color:#3a3a3a\"><strong style=\"color:#8a1524\">For Research Use Only (RUO).<\/strong> This product is sold strictly for laboratory research use only. <strong>Not for human or veterinary consumption, therapeutic, or diagnostic use.<\/strong> Any pharmacological, therapeutic, cosmetic, or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>EvaEZ\u2122 Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen\u00ae dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. In the presence of DNA polymerase activity, the primer will be extended to form [&hellip;]<\/p>\n","protected":false},"featured_media":714830,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[1638],"product_cat":[1720,1731],"product_tag":[],"class_list":{"0":"post-715947","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-biotium","7":"product_cat-biotium-other-qpcr-reagents","8":"product_cat-biotium-qpcr-reagents","10":"first","11":"instock","12":"taxable","13":"shipping-taxable","14":"purchasable","15":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/715947","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/714830"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=715947"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=715947"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=715947"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=715947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}