{"id":148807,"date":"2025-08-05T10:20:02","date_gmt":"2025-08-05T10:20:02","guid":{"rendered":"https:\/\/immunomart.com\/product\/4-hne4-hydroxynonenal-elisa-kit\/"},"modified":"2026-04-12T19:47:12","modified_gmt":"2026-04-12T19:47:12","slug":"4-hne4-hydroxynonenal-elisa-kit","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/4-hne4-hydroxynonenal-elisa-kit\/","title":{"rendered":"4-HNE(4-Hydroxynonenal) ELISA Kit"},"content":{"rendered":"<p>This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and [&hellip;]<\/p>\n","protected":false},"featured_media":148809,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[873],"product_cat":[1226],"product_tag":[],"class_list":{"0":"post-148807","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-c2","7":"product_cat-general-ruo-elisa","8":"pa_size-48t","9":"pa_size-96t","11":"first","12":"instock","13":"sale","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/148807","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/148809"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=148807"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=148807"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=148807"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=148807"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}