{"id":144114,"date":"2025-08-05T05:20:04","date_gmt":"2025-08-05T05:20:04","guid":{"rendered":"https:\/\/immunomart.com\/product\/dog-pro-anppro-atrial-natriuretic-peptide-elisa-kit\/"},"modified":"2026-04-12T19:41:57","modified_gmt":"2026-04-12T19:41:57","slug":"dog-pro-anppro-atrial-natriuretic-peptide-elisa-kit","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/dog-pro-anppro-atrial-natriuretic-peptide-elisa-kit\/","title":{"rendered":"Dog Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit"},"content":{"rendered":"<p>The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Dog Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) [&hellip;]<\/p>\n","protected":false},"featured_media":144116,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[873],"product_cat":[1199],"product_tag":[],"class_list":{"0":"post-144114","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-c2","7":"product_cat-dog-ruo-elisa","8":"pa_size-48t","9":"pa_size-96t","11":"first","12":"instock","13":"sale","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/144114","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/144116"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=144114"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=144114"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=144114"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=144114"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}