{"id":136921,"date":"2025-08-05T03:01:03","date_gmt":"2025-08-05T03:01:03","guid":{"rendered":"https:\/\/immunomart.com\/product\/chicken-baff-cd257b-cell-activating-factor-elisa-kit\/"},"modified":"2026-04-12T19:33:43","modified_gmt":"2026-04-12T19:33:43","slug":"chicken-baff-cd257b-cell-activating-factor-elisa-kit","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/chicken-baff-cd257b-cell-activating-factor-elisa-kit\/","title":{"rendered":"Chicken BAFF\/CD257(B-Cell Activating Factor) ELISA Kit"},"content":{"rendered":"<p>The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken BAFF\/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken BAFF\/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken BAFF\/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Chicken BAFF\/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken BAFF\/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken BAFF\/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) [&hellip;]<\/p>\n","protected":false},"featured_media":136928,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[873],"product_cat":[1208],"product_tag":[],"class_list":{"0":"post-136921","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-c2","7":"product_cat-chicken-ruo-elisa","8":"pa_size-48t","9":"pa_size-96t","11":"first","12":"instock","13":"sale","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/136921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/136928"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=136921"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=136921"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=136921"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=136921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}