{"id":100775,"date":"2025-08-04T13:41:17","date_gmt":"2025-08-04T13:41:17","guid":{"rendered":"https:\/\/immunomart.com\/product\/human-hsp-70-hspa9heatshock-protein-70-elisa-kit\/"},"modified":"2026-04-12T17:05:29","modified_gmt":"2026-04-12T17:05:29","slug":"human-hsp-70-hspa9heatshock-protein-70-elisa-kit","status":"publish","type":"product","link":"https:\/\/immunomart.com\/product\/human-hsp-70-hspa9heatshock-protein-70-elisa-kit\/","title":{"rendered":"Human HSP-70\/HSPA9(HeatShock Protein 70) ELISA Kit"},"content":{"rendered":"<p>The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70\/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP-70\/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP-70\/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Human HSP-70\/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70\/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP-70\/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) [&hellip;]<\/p>\n","protected":false},"featured_media":100777,"template":"","meta":{"_acf_changed":false,"ep_exclude_from_search":false},"product_brand":[873],"product_cat":[1195],"product_tag":[],"class_list":{"0":"post-100775","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-c2","7":"product_cat-human-ruo-elisa","8":"pa_size-48t","9":"pa_size-96t","11":"first","12":"instock","13":"sale","14":"taxable","15":"shipping-taxable","16":"purchasable","17":"product-type-variable"},"acf":[],"_links":{"self":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product\/100775","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media\/100777"}],"wp:attachment":[{"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/media?parent=100775"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_brand?post=100775"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_cat?post=100775"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/immunomart.com\/wp-json\/wp\/v2\/product_tag?post=100775"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}