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DPA/Terbium for membrane fusion assay

Name: DPA/Terbium for membrane fusion assay | Biotium Reagent | Immunomart
Brand: Biotium
SKU: BT-80104
Price: 100 USD
Availability: InStock

SKU BT-80104 Categories Cytosolic Tracers, Dextrans & Other Fluid Phase Tracers Brand: Biotium

100 USD

Only 1000 item(s) left in stock.

For Research Use Only (RUO). This product is sold strictly for laboratory research use. Not for human or veterinary consumption, therapeutic, or diagnostic use.Intended for use by research organizations and laboratories only; not for patients or for personal use.

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Product Description

– The principle of DPA/Tb³ for vesicle fusion assay is based on the fact that contact of the chelator dipicolinic acid (DPA) with terbium (III) forms an instant Tb³-DPA complex that is ~10,000 times more fluorescent than free Tb³. In the assay, separate vesicle populations are loaded with 2.5 mM TbCl3 in 50 mM sodium citrate, or 50 mM DPA in 20 mM NaCl. Fusion of the two types of vesicles results in fluorescence increase at 490 nm or 545 nm, with excitation at 276 nm. Each set of product contains 1 g terbium trichloride and 1 g DPA in two separate vials. Please also see SDIP/Europium for membrane fusion assay, which results in intense red fluorescence upon complex formation.

&lamba;Ex/&lamba;Em (DPA/Tb³ complex) = 276/490 and 545 nm
DPA and Terbium are white solids and readily soluble in water
Store both reagents at room temperature
MW of DPA: 167.12
CAS#: 499-83-2
MW of Terbium: 265.3
CAS#:13798-24-8

For Research Use Only (RUO). This product is sold strictly for laboratory research use only. Not for human or veterinary consumption, therapeutic, or diagnostic use. Any pharmacological, therapeutic, [cosmetic chemistry research literature], or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.

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Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.